Our goal at MiGS is to provide high quality sequencing data at a price and turnaround rate that is unmatched in the industry. We focus specifically on small microbial genomes, which require ~1000x fewer reads than human genomes and for which library preparation is cost-prohibitive using standard commercial protocols. To reduce costs significantly, we employ a method that dilutes standard reagents appropriate to the small genomes, utilizes PCR amplification to permit low DNA input and optimally pools these genomes onto single runs of our Illumina NextSeq500 genome sequencer. By focusing on small genomes, we are able to use a single work flow that efficiently processes all incoming samples and allows us to deliver data within 14 days of receiving DNA samples.
The science behind our library preparation method has been published in this peer-reviewed article.
Samples are received and immediately frozen until the library preparations begins. All samples are normalized to the same concentration and enzymatically fragmented using an Illumina tagementation enzyme. Unique indices are attached to each pool of fragmented genomic DNA using PCR and the resulting barcoded pools are combined to multiplex on an Illumina NextSeq 500 flow cell.
Our sequencing work produces 2 x 151bp paired end reads that are demultiplexed to individual samples and distributed to you via Box. The folders within your link will indicate the date of the sequencing run and the index combination that was used for your sample. Within the folder, you will find 4 files named after your sample name. The R1 and R2 .fastq files will contain the forward and reverse reads for your sample, and the I1 and I2 .fastq files will contain the reads for the indices. The index reads can be helpful for custom quality filtering application, however most of our customers do not use these files in thier analysis pipeline.
Your data will be available to you on Box for a period of 6 months.